janewinslet
A Biology Researcher
MiRNA Sequencing Service - Creative Biogene IntegrateRNA. IntegrateRNA is a leading global biotechnology company, and is dedicated to providing a wide range of services for RNA discovery, analysis, and related bioinformatics analysis.
Our experts have examined thousands of different RNA data sets, which gives us a unique insight into RNA research. To support your research, we offer complete microRNA sequencing services including RNA preparation and quality control, library construction, sequencing, and comprehensive data analysis, producing the results needed to rapidly advance biological and biomedical research. MicroRNAs (miRNA) are small, non-coding RNA molecules that function broadly as negative regulators of gene expression to control a wide range of cellular processes. Thousands of miRNAs have been identified in humans, but only a small fraction of them have demonstrated functional roles. MiRNA Services - Creative Biogene IntegrateRNA. IntegrateRNA has developed a complete workflow for your miRNA basic research, biomarker discovery and preclinical trials, enhanced with the latest and most enabling technologies.
We provide customized, one-stop solution, from miRNA discovery, confirmation, functional analysis, target validation, mechanism analysis to in vivo experiment. Our innovative portfolio of miRNA analysis solutions will help you gain more insights and make the most impact with your studies. MicroRNAs (miRNAs) are short, highly conserved small noncoding RNA molecules naturally occurring in the genomes of plants and animals. They are 17–27 nucleotides long and regulate posttranscriptional mRNA expression, typically by binding to the 3' untranslated region (3'-UTR) of the complementary mRNA sequence, resulting in translational repression and gene silencing.
Gene Silencing - Creative Biogene Baseceuticals. As one of the top companies in genetic modification filed, Baseceuticals are proud to provide comprehensive gene silencing services for customers worldwide.
Based on the outstanding platform, our experienced technical staffs have developed best-in-class technologies to complete a variety of gene silencing projects with satisfaction guaranteed. We mainly use RNA interference and antisense oligonucleotide methods for gene silencing to meet your various needs. Our research team will cooperate with you closely, assisting you to achieve the research goal. Gene silencing is a natural cellular phenomenon, implements blocking by causing degradation of the intermediate RNA form before the RNA code can be translated into a protein. The most commonly used methods of gene silencing are RNA interference (RNAi) and antisense oligonucleotide (ASO) technologies. Fig.1 Gene silencing mechanisms of siRNA and miRNA Fig.2 Antisense oligonucleotides (ASOs) vs RNA interference (RNAi) References: Lam, J.
CRISPR/Cas9 Service - Creative Biogene Baseceuticals. As a leading provider of gene editing service, Baseceuticals is proud to offer comprehensive CRISPR/Cas9 services.
IGF1R Gene Editing - Creative Biogene CRISPR/Cas9. IGF1R Gene Editing The Insulin-like Growth Factor Receptor (IGF1R), also known as CD221/JTK13, is a transmembrane receptor found on the surface of human cells and belongs to the large class of tyrosine kinase receptors, mediates the effects of IGF-1.
IGF1R is activated by hormone called insulin-like growth factor 1 (IGF-1), a polypeptide protein hormone similar to insulin in molecular structure, and by the related growth factor IGF-2. IGF1R is a hetero-tetrameric protein with two identical extracellular α-subunits and two transmembrane β-subunits. The extracellular domain contains a cysteine-rich IGF-binding site. cGMP E.coli & Yeast Fermentation - Creative Biogene Microbiosci. Microbiosci offers custom GMP (cGMP) contract manufactured E.coli and Yeast at various scales for research and clinical investigations.
As the leading CDMOs, Microbiosci has good expertise and capabilities in the development and optimization of microbial-based processes as well as scale up for manufacture. E.coli, which is fast and easy to culture, is one of the most popular expression platforms. The variety molecular technique make the E.coli easy to be modified and transformed. Fig. E.coli. Replication-Competent Adenovirus Testing - Biogene Qvirus Platform. Replication-defective adenovirus vectors are deficient in early viral gene E1 and are generally grown in cell lines such as HEK-293, which contain and express an integrated copy of E1 for the purpose of complementing the defective adenoviral genome.
Although the host cell E1 gene is required for complementation, its presence can also be problematic because recombination between vector and host cell genomes may lead to the reacquisition of E1 by the vector and the formation of replication-competent adenoviruses (RCAs). This problem has long been recognized, and approaches, such as the minimization of the sequence homology between the adenovirus genes inserted into the host chromosome DNA and viral sequences remaining in the 5’ end of the vector genome, have been taken to reduce recombination and RCA formation. However, in the clinical setting, it is important to ensure that the vector dose given to patients contains no more than one RCA infectious particle per patient dose. Reference 1. Industrial Enzymes Fermentation - Creative Biogene Microbiosci. Microorganisms are favored sources for industrial enzymes owing to easy availability and fast growth rate.
Genetic changes via recombinant DNA technology can easily be done on microbial cells for elevated enzyme production. Production of microbial enzymes is a necessary event in the industrial field, due to the high and superior performances of enzymes from different microbes, which work well under a broad range of varied physical and chemical conditions. Besides, microbial enzymes are used in the treatment of various health disorders associated with deficiency of human enzymes caused by genetic problems. EphA2 Gene Editing - Creative Biogene CRISPR/Cas9. EphA2 Gene Editing Ephrin type-A receptor 2 (EphA2) is a 130 kDa glycoprotein receptor and belongs to the largest EPH family receptor tyrosine kinases.
The EphA2 protein structure has an intracellular kinase domain, and an extracellular domain with an Ephrin binding domain and two fibronectin type III repeats. Adenovirus Production Services - Biogene Qvirus Platform. Adenovirus is a replication-defective adenoviral vector system that widely used for gene delivery in a variety of cell types.
The vectors are predominantly nonenveloped, nonintegrating, episomal, double-stranded DNA viruses. With a strong safety record, adenoviruses and recombinant adenoviral vectors have been widely used for gene function studies and human gene therapy. Recombinant adenoviruses that cannot replicate are attractive vectors for gene delivery for the following reasons: 1. Adenovirus has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues. 2.
Figure 1. Oligo Pools. Oligo Pools Creative Biogene is a leading biotechnology company which has the expertise and ability in providing oligo pool design and synthesis service for customers worldwide. Creative Biogene's sophisticated equipments, advanced technologies and highly experienced staffs are available to help you carry out your specific project as cost effectively, quickly and simply as possible, without compromising on quality. Although, as we all know, the cost of DNA synthesis has dropped by five orders of magnitude in the past decade, its applications in many other fields is still very expensive, such as exon trapping and targeted regions trapping. Exon trapping and targeted regions trapping are commonly used in the second generation sequencing method. However, the needs of thousands of primers have made the process of work difficult. Creative Biogene's custom oligo pool design and synthesis service possesses unparalleled advantages: Reference:
Herpes Simplex Virus (HSV) Service. Herpes Simplex Virus (HSV) Service Creative Biogene is a world leader in the area of virus engineering and packaging. With the deep knowledge on the genetics and molecular biology of HSV, along with our state-of-the-art facilities, scientists at Creative Biogene have developed an excellent HSV production platform. Herpes simplex virus (HSV) is an enveloped, large linear and double-stranded DNA (~150 kb) virus in the family Herpesviridae. Custom Knockin Cell Line Generation - Creative Biogene CRISPR/Cas9. Knockin Cell Line Generation CRISPR/Cas9 PlatformCB provides one stop custom knock-in cell line generation service, including point mutation and gene insertion. With non-stop exploration of technology, we have developed an excellent platform for gene editing. Based on the platform, our expert staff has succeeded in dozens of knock-in cell line generation projects, including stem cells, tumor cells and even difficult-to-handle cells.
At CRISPR/Cas9 PlatformCB, a full cell line generation service is offered from sgRNA design/construction to final cell line generation/verification. CRISPR system is a novel tool for precise gene editing. Fig1. CRISPR Off-Target Effects Analysis - Creative Biogene CRISPR/Cas9. CRISPR Off-Target Effects Analysis CRISPR/Cas9 PlatformCB provides an off-target prediction and analysis service for CRISPR/Cas9-mediated gene editing. With years of experiences, our scientists are expert in application of CRSIPR/Cas9 system. Our scientist team will provide reliable and professional off-target testing services for our clients. As a leader in gene editing, CRISPR/Cas9 PlatformCB offers trusted analysis results with high quality in a competitive price.
Cas9, which is a nuclease, can be recruited to the specific site by guide RNA and then cleaves DNA. Industrial Enzymes Fermentation - Creative Biogene Microbiosci. Microbial Fuel Cell Service - Creative Biogene Microbiosci. The transfer of electrons among molecules is essential to all organisms, microbes are no exception, for this process generates energy by which they need to survive. While some microorganisms living in oxygen-deprived environments have evolved a unique form of breathing that involves excreting and pumping out electrons, have the potential to act as electricity-producing components that can be used in microbial fuel cells (MFCs), which can be put into use of contributing energy.
But not just limit to that, by the energy harvesting process of microbial anaerobic digestion, MFCs is a brilliant tool for wastewater treatment, meanwhile, pathogens can also be reduced, which is a quite promising field in ecological restoration. Moreover, MFCs can work as biosensors to measure the solute concentration of wastewater, online monitoring can be realized as to BOD, COD, DO, toxics, etc. Figure 1. Baculovirus Services - Biogene Qvirus Platform. Baculovirus has been widely used as a gene expression tool for over 30 years based on its ability to express high levels of proteins in insect cells.
Along with the progress of baculovirus research, recombinant baculoviruses have been demonstrated to be capable of entering into various mammalian cells and of expressing foreign genes under the control of mammalian promoters without any viral replication; therefore, they have been applied for gene delivery into mammalian cells. Oncolytic Adenovirus Service - Biogene Qvirus Platform. Having a broad tissue tropism and the capability of infecting a large variety of dividing and non-dividing cells, adenoviruses belong to the most intensively studied viral vectors for gene therapy, and oncolysis. In addition to their potent lytic activity, adenoviruses have several striking advantages for pharmacologic application.
The adenoviral genome is easily accessible for genetic manipulation and the well-understood adenovirus biology provides for ample opportunities of tailored gene modifications. With regard to manufacturing, adenoviruses can be produced at high titers and particles are of excellent physicochemical stability. Regarding safe clinical applicability, tumor-selective replication has been considered as an important precondition to prevent excess damage to non-target, healthy tissue. Oncolytic adenoviruses selectively replicate in and lyse cancer cells and are therefore commonly used vectors in clinical trials for cancer gene therapy. Figure 1. References1. Lentivirus Service. Lentivirus Service Brief Introduction. Green Fluorescent Protein. Official Full Name Green Fluorescent Protein Background. Mutagenesis service. Creative Biogene is a leading biotechnology company which has the expertise in providing gene mutagenesis service for customers.
Premade Adenovirus Particles. AAV Service - Creative Biogene. Creative Biogene possesses the world-class production facility and manufacture rAAV vectors with the highest possible stringency, resulting in an extremely pure, high quality product every time. Custom Viral Service - Creative Biogene. Aptamers. Aptamers Aervice. Adenoviral vector.