Study of Multidrug Resistant Strain of K. Oxytoca Abstract Klebsiella are opportunistic pathogens that cause a wide spectrum of severe diseases. The aim of the present study was to investigate the impact of biofield treatment on multidrug resistant strain of K. oxytoca with respect to antibiogram pattern along with biochemical study and biotype number. Keywords: Klebsiella oxytoca ; Multidrug resistant; Antibiogram; Biofield treatment; Biochemical reactions; Biotyping Introduction Klebsiella oxytoca (K. oxytoca) is a Gram-negative pathogen, cylindrical rod shaped, non-motile in nature, and belongs to Enterobacteriaceae family. Biofield is a cumulative outcome of electric and magnetic field energy, exerted by the human body. Materials and Methods Experimental design and biofield treatment MDR clinical strain of K. oxytoca was collected from stored stock cultures of clinical sample in Microbiology Lab, Hinduja Hospital, Mumbai. Evaluation of antimicrobial susceptibility assay Biochemical reaction study Identification by biotype number 1.
Antimicrobial Susceptibility, Biochemical Characterization and Molecular Typing of Biofield Treated Klebsiella pneumoniae - Trivedi Science Abstract: Pathogenic isolates of Klebsiella pneumoniae (K. pneumoniae), particularly the extended-spectrum β-lactamase (ESBL) producing strains, are mostly associated with the failure of antibiotic therapy in nosocomial infections. The present work was designed to evaluate the impact of Mr. Trivedi’s biofield energy treatment on phenotypic and genotypic characteristics of K. pneumoniae. Keywords: Klebsiella pneumoniae; Biofield energy treatment; Antibiogram, Biochemical reactions, Polymorphism; Random Amplified Polymorphic DNA. Abbreviations: CAM: Complementary and Alternate Medicine; NHIS: National Health Interview Survey; NCHS: National Center for Health Statistics; ATCC: American Type Culture Collection; MIC: Minimum Inhibitory Concentration; MEGA: Molecular Evolutionary Genetics Analysis; NBPC 30: Negative Breakpoint Combo Panel 30; RAPD: Random Amplified Polymorphic DNA; PCR: Polymerase chain reaction; ESBL: Extended Spectrum β-Lactamase Introduction Materials and Methods Biotype number
Impact of Biofield Treatment on Enterobacter Aerogenes Abstract: Enterobacter aerogenes (E. aerogenes) has been reported as the versatile opportunistic pathogen associated with the hospital infections worldwide. The aim of the study was to determine the impact of Mr. Trivedi’s biofield energy treatment on multidrug resistant clinical lab isolates (LSs) of E. aerogenes. The MDR isolates of E. aerogenes (i.e., LS 45 and LS 54) were divided into two groups, i.e., control and treated. Samples were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study, and biotype number using MicroScan Walk-Away® system, on day 10 after the biofield treatment. Keywords: Enterobacter aerogenes; Multidrug resistant; Antimicrobial susceptibility; Biofield treatment; Biochemical reactions; Biotyping Introduction Enterobacter is a genus of Gram-negative, rod shaped, facultative anaerobic, and non-spore forming microbes of family Enterobacteriaceae. Materials and Methods Inoculum preparation Biofield treatment Results
Effect of Biofield Treatment on Staphylococcus Species Abstract: Antimicrobial resistance is a global health issue in the developing countries. This study was carried out to evaluate the impact of Mr. Trivedi’s biofield energy treatment on multidrug resistant (MDR) clinical lab isolates (LSs) of Staphylococcus species viz. Keywords Staphylococcus haemolyticus, Staphylococcus epidermidis, Staphylococcus aureus, Biofield Energy Treatment, Multidrug-Resistant, Antibiogram, Biotyping 1. Staphylococci are members of the family Staphylococcaceae. Biofield is the name given to the electromagnetic field that permeates and surrounds the living organisms. 2. 2.1. MDR clinical lab isolates (LSs) of S. haemolyticus (LS 18), S. epidermidis (LS 21) and S. aureus (LS 30) were obtained from stored stock cultures in Microbiology Lab, Hinduja Hospital, Mumbai. 2.2. Treatment groups of each strain, in sealed pack were handed over to Mr. 2.3. 2.4. 2.5. 3. 3.1. Table 1. 3.2. Table 3. Table 4. 4. Acknowledgements References Download the original manuscript
Antimicrobial & Biotyping Analysis of Escherichia Coli Abstract Escherichia coli (E. coli) infections are the major health concern, as it causes infections in human mainly in urinary tract, ear, and wound infections. The present study evaluates the impact of biofield energy treatment on E. coli regarding antimicrobial sensitivity assay, biochemical study and biotype number. Four multidrug resistant (MDR) clinical lab isolates (LSs) of E. coli (LS 12, LS 13, LS 42, and LS 51) were taken in two groups i.e. control and treated. Keywords: Escherichia coli, Biofield Energy Treatment, Multidrug-Resistant, Antibiogram, Biochemical, Biotyping 1. Escherichia coli (E. coli) is a Gram-negative, rod shape, and facultative anaerobic pathogen linked with communityassociated as well as nosocomial infections. Alternative medicine remains alternative due to their serious challenges against mainstream biomedical paradigm, as it requires a new framework. 2. 2.1. The MDR clinical lab isolates of E. coli (i.e. 2.2. 2.3. 3. 3.1. 3.2. Table 4. 4. Abbreviations
Effect of Biofield Energy Treatment on Streptococcus group B: A Postpartum Pathogen - Trivedi Science Abstract: Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial septicemia and neonatal meningitis. The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr. Keywords: Streptococcus agalactiae group B; Biofield treatment; Minimum inhibitory concentration; Biochemical reaction; Biotype Abbreviations: CLSI: Clinical and laboratory standards institute; GBS: Group B Streptococci; CAMP: Christie-Atkins-Munch-Petersen; CDC: Centers for Disease Control and Prevention; ACOG: American College of Obstetricians and Gynecologists; AAP: American Academy of Pediatrics; MIC: Minimum inhibitory concentration; ATCC: American Type Culture Collection; PBPC 20: Positive Breakpoint Combo 20 Introduction Materials and Methods Experimental design Two ATCC samples A and B of S. agalactiae gr. Biofield treatment strategy 1.
Influence of Human Biofield on Klebsiella oxytoca Abstract Klebsiella oxytoca (K. oxytoca) is a Gram-negative microbe generally associated with community and hospitalacquired infections. Due to its clinical significance, we evaluated the effect of biofield treatment on phenotype and biotype characteristics of K. oxytoca (ATCC 43165). The study was performed into three groups i.e. C (control), T1 (treatment, revived); and T2 (treatment, lyophilized). Keywords : Antimicrobials; Biochemicals; Biofield treatment; Biotype; Klebsiella oxytoca Introduction Microorganisms like bacteria, viruses, fungi, and parasites are continuously acquiring the resistance against existing antimicrobials that possess a major global threat to public health. Klebsiella oxytoca (K. oxytoca) is a rod-shaped, nonmotile, Gramnegative bacterium with a prominent polysaccharide capsule, which provides a resistance against host defense mechanisms. Mr. Materials and Methods Study design Investigation of antimicrobial susceptibility of microorganism Results Discussion 1.
Impact Of Biofield Treatment On Salmonella Paratyphi A | Trivedi Science Abstract Enteric fever is a major global problem. Emergence of antimicrobial resistance threatens to render current treatments ineffective. Keywords: S. paratyphi A; Paratyphoid fever; Antimicrobial susceptibility; Biofield treatment; Biochemical reaction; Biotype Abbreviations MIC: Minimum Inhibitory Concentration; ATCC: American Type Culture Collection; NBPC 30: Negative Breakpoint Combo 30 Introduction Enteric fever is a major public health problem in India. Fluoroquinolone derivatives like ciprofloxacin, nalidixic acid etc. are extensively used to combat paratyphoid fever in most parts of world. Harold Saton Burr, had performed the detailed studies on the correlation of electric current with physiological process and concluded that every single process in the human body had an electrical significance [10]. Materials and Methods S. paratyphi A, American Type Culture Collection (ATCC 9150) strains were procured from MicroBioLogics, Inc., USA, in two sets A and B. Experimental design Gr. 1.