Klebsiella Pneumoniae Biochemical Characteristics Abstract: Pathogenic isolates of Klebsiella pneumoniae (K. pneumoniae), particularly the extended-spectrum β-lactamase (ESBL) producing strains, are mostly associated with the failure of antibiotic therapy in nosocomial infections. The present work was designed to evaluate the impact of Mr. Trivedi’s biofield energy treatment on phenotypic and genotypic characteristics of K. pneumoniae. The strain of K. pneumoniae bearing ATCC 15380 (American Type Culture Collection) was procured from the Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to Mr. Keywords: Klebsiella pneumoniae; Biofield energy treatment; Antibiogram, Biochemical reactions, Polymorphism; Random Amplified Polymorphic DNA. Abbreviations: Introduction The increased medical practice for antibiotic usage creates selection pressure and results emergence of nosocomial pathogens. Materials and Methods Biofield treatment modalities Biochemical studies Biotype number Conclusion 1.
Providencia Rettgeri Biochemical Reactions Analysis Abstract Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The current study was designed to investigate the effect of Mr. Trivedi’s biofield energy treatment on P. rettgeri in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. The lyophilized strain of P. rettgeri (ATCC 9250) was divided into two parts, Group (Gr.) I: control and Gr. Keywords: Providencia rettgeri, Antimicrobial Sensitivity, Minimum Inhibitory Concentration, Biofield Treatment,Biochemical Reaction, Biotype 1. Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. 2. The strain P. rettgeri, bearing the American Type Culture Collection (ATCC 9250) strain was procured from MicroBioLogics, Inc., USA. 2.1. Group I: ATCC strain in the lyophilized state was considered as control. 2.2. 2.3. 2.4.
Study of Multidrug Resistant Strain of K. Oxytoca Abstract Klebsiella are opportunistic pathogens that cause a wide spectrum of severe diseases. The aim of the present study was to investigate the impact of biofield treatment on multidrug resistant strain of K. oxytoca with respect to antibiogram pattern along with biochemical study and biotype number. Clinical lab isolate of K. oxytoca was divided into two groups i.e. control and treated. Control group remain untreated and treated group was subjected to Mr. Keywords: Klebsiella oxytoca ; Multidrug resistant; Antibiogram; Biofield treatment; Biochemical reactions; Biotyping Introduction Klebsiella oxytoca (K. oxytoca) is a Gram-negative pathogen, cylindrical rod shaped, non-motile in nature, and belongs to Enterobacteriaceae family. Biofield is a cumulative outcome of electric and magnetic field energy, exerted by the human body. Materials and Methods Experimental design and biofield treatment Evaluation of antimicrobial susceptibility assay Biochemical reaction study Results and Discussion
Antimycobacterial Susceptibility Testing of Antimicrobials Abstract The aim was to evaluate the impact of biofield treatment modality on mycobacterial strains in relation to antimycobacterials susceptibility. Mycobacterial sensitivity was analysed using 12 B BACTEC vials on the BACTEC 460 TB machine in 39 lab isolates (sputum samples) from stored stock cultures. Two American Type Culture Collection (ATCC) strains were also used to assess the minimum inhibitory concentration (MIC) of antimicrobials (Mycobacterium smegmatis 14468 and Mycobacterium tuberculosis 25177). Rifampicin, ethambutol and streptomycin in treated samples showed increased susceptibility as 3.33%, 3.33% and 400.6%, respectively, as compared to control in extensive drug resistance (XDR) strains. Keywords: Antimycobacterial susceptibility; Biofield treatment;MDR-TB; XDR-TB; BACTEC 460/MGIT-TB; MIC Introduction Global tuberculosis control is facing major challenges now days. Mr. Materials and Methods Biofield Treatment Strategy and Experimental Design Results and Discussion References
Energy Treatment and Shigella Sonnei Characteristics Abstract: Shigella sonnei (S. sonnei) is a non-motile, rod shape, clinically significant, Gram-negative bacterium. It is commonly associated with dysentery (shigellosis). Recently, resistance to third and fourth generation cephalosporins and fluoroquinolones has been reported in S. sonnei. In the present study, we assessed the effect of biofield treatment on phenotyping and genotyping characteristic of S. sonnei (ATCC 9290). The lyophilized samples of S. sonnei were divided in three groups (G): G-I (control, revived), G-II (treatment, revived), and G-III (treatment, lyophilized). Keywords: Antimicrobial susceptibility; Biofield treatment; 16S rDNA gene sequencing; Shigella sonnei Introduction Development of antimicrobial resistance in several microbes like bacteria, viruses, fungi, or in parasites has been reported globally in the recent few decades. Materials and Methods Biofield treatment Investigation of antimicrobial susceptibility of S. sonnei Biochemical studies Biotype number Results 1.
Energy Treatment Impact on Antifungal Sensitivity Assay Abstract Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated the impact of biofield treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern. Each fungal sample was divided into three parts: C, control; T1, treatment (revived); T2 treatment (lyophilized). Keywords: Fungi; Amphotericine B; Fluconazole; Flucytosine; Itraconazole Introduction The kingdom fungi includes yeast, mold, and mushrooms that have multiple applications like fermentation of sugar and bear; productions of antibiotics and enzymes; as biological pesticide; and also consumed as food products like mushrooms, morels, and truffles. Energy is the property of matter that neither can be created nor destroyed, however it can be transmitted to other substances by changing into different forms. Materials and Methods Biofield treatment Investigation of antifungal sensitivity and fungal strain identification Results Antifungal sensitivity
Quality of Beef Extract & Meat Infusion Powder- Energy Impact The present research work investigated the influence of bio-field treatment on two common flavoring agents used in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size analysis, surface area analysis, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The FT-IR results showed disappearance of triglycerides peaks in both the treated powders as compared to control. Keywords : Beef extract powder; Meat infusion powder; Bio-field treatment; Fourier transform infrared spectroscopy; X-ray diffraction; Particle size analysis; Surface area analysis; Differential scanning calorimetry; Thermo gravimetric analysis Beef is known to have excellent nutritional value and it has been widely consumed in many countries. Coronory heart disease (CHD) is the main cause of death in western countries. Mr. Discussion Conclusion
Antimicrobial & Biotyping Analysis of Escherichia Coli Abstract Escherichia coli (E. coli) infections are the major health concern, as it causes infections in human mainly in urinary tract, ear, and wound infections. The present study evaluates the impact of biofield energy treatment on E. coli regarding antimicrobial sensitivity assay, biochemical study and biotype number. Four multidrug resistant (MDR) clinical lab isolates (LSs) of E. coli (LS 12, LS 13, LS 42, and LS 51) were taken in two groups i.e. control and treated. After treatment, above mentioned parameter were evaluated on day 10 in control and treated samples using MicroScan Walk-Away® system. The antimicrobial sensitivity assay was reported with 46.67% alteration (14 out of 30 tested antimicrobials) in treated group of MDR E. coli isolates. Keywords: Escherichia coli, Biofield Energy Treatment, Multidrug-Resistant, Antibiogram, Biochemical, Biotyping 1. 2. 2.1. The MDR clinical lab isolates of E. coli (i.e. 2.2. 2.3. 3. 3.1. 3.2. Table 4. 4. Abbreviations Acknowledgements
Gluten Hydrolysate - Physical and Thermal Properties Modification Abstract The objective of present study was to study the effect of biofield treatment on physical and thermal properties of gluten hydrolysate (GH) and ipomoea macroelements (IM). The study was performed in two groups (control and treated). The control group remained as untreated, and biofield treatment was given to treated group. The control and treated GH and IM were characterized by particle size analysis, surface area analysis, X-ray diffraction (XRD), Differential scanning calorimetry (DSC), and Thermogravimetric analysis (TGA). Particle size results of treated GH showed that d50 (average particle size) was decreased by 3.15% and d99 (size exhibited by 99% of powder particles) by 18.40% as compared to control GH sample. Keywords: Gluten hydrolysate; Ipomoea macroelements; Biofield treatment; Particle size; Surface area; XRD; DSC; TGA Introduction Experimental The gluten hydrolysate (GH) and ipomoea macroelements (IM) powder were procured from HiMedia Laboratories Pvt. Characterization
Impact of Energy Treatment on Lithium Powder Abstract Lithium has gained extensive attention in medical science due to mood stabilizing activity. The objective of the present study was to evaluate the impact of biofield treatment on physical, atomic, and thermal properties of lithium powder. Keywords: Biofield treatment; Lithium; X-ray diffraction; Differential scanning calorimetry; Thermogravimetric analysis-differential thermal analysis; Scanning electron microscopy; Fourier transform infrared spectroscopy Introduction Lithium is highly reactive, light metal, which is commonly found in various foods such as grains, vegetables, mustard, kelp, and fish blue corn etc. Harold Saton Burr had performed the detailed studies on the correlation of electric current with physiological process and concluded that every single process in the human body had an electrical significance [8]. which states that the moving charge produces magnetic fields in surrounding space [9,10]. used to measure the biofield of human body [11]. G=kλ/(bCosθ), 1.
Chloramphenicol & Tetracycline - Spectroscopic Analysis Abstract Objective: Chloramphenicol and tetracycline are broad-spectrum antibiotics and widely used against variety of microbial infections. Nowadays, several microbes have acquired resistance to chloramphenicol and tetracycline. The present study was aimed to evaluate the impact of biofield treatment for spectroscopic characterization of chloramphenicol and tetracycline using FT-IR and UV-Vis spectroscopy. Methods:The study was performed in two groups (control and treatment) of each antibiotic. Results: FT-IR spectrum of treated chloramphenicol exhibited the decrease in wavenumber of NO2 from 1521 cm-1 to 1512 cm-1 and increase in wavenumber of C=O from 1681 cm-1 to 1694 cm-1 in acylamino group. Conclusion: Based on FT-IR spectroscopic data, it is speculated that due to increase in bond strength and conjugation effect after biofield treatment, the chemical stability of both the drugs might be increased as compared to control. Introduction: Materials and Methods Study design Conclusion 1.
Spectral Properties of Paracetamol - Biofield Energy Treatment Abstract Paracetamol and piroxicam are non-steroidal anti-inflammatory drugs (NSAIDs), widely used in pain and inflammatory diseases. The present study aimed to evaluate the impact of biofield treatment on spectral properties of paracetamol and piroxicam. The study was performed in two groups (control and treatment) of each drug. Keywords: Paracetamol; Piroxicam; Biofield treatment; Fourier transform infrared spectroscopy; Ultraviolet spectroscopy Introduction Paracetamol [N-(4-Hydroxyphenyl) ethanamide] acetaminophen (in United States) is an analgesic and antipyretic drug, widely used for pain (back and neck) and fever for approximately 50 years and has relatively few side effects [1,2].However, it is ineffective in the pain originating from smooth muscle spasm in internal organs. Piroxicam is N-heterocyclic carboxamide of 1,2 benzothiazine 1,1 dioxide. Mr. Materials and Methods Study design FT-IR Spectroscopic characterization UV-Vis Spectroscopic analysis References 1.
Physical & Thermal Characteristics of Selenium Abstract Selenium (Se) is an essential trace element, and its deficiency in the humans leads to increase the risk of various diseases, such as cancer and heart diseases. The objective of this study was to investigate the influence of biofield energy treatment on the physical and thermal properties of the selenium powder. The selenium powder was divided into two parts denoted as control and treated. The Control part was remained as untreated and treated part received Mr. Keywords: Biofield Energy Treatment, Selenium Powder, X-ray Diffraction, Thermogravimetric Analysis – Differential Thermal Analysis, Differential Scanning Calorimetry,Fourier Transform Infrared 1. The importance of selenium (Se) in human is well established, and its deficiency has caused serious diseases such as cancer and heart disease [1]. It is well established that all atoms are in motion, which contain significant amount of energy. 2. The selenium powder was purchased from Alpha Aesar, Hyderabad, India. 2.1. 2.2. 3.
Physicochemical Properties of Hydroxyethyl Cellulose Abstract Cellulose based polymers have shown tremendous potential as drug delivery carrier for oral drug delivery system (DDS). Hydroxyethyl cellulose (HEC) and hydroxypropyl cellulose (HPC) are widely explored as excipients to improve the solubility of poorly water soluble drugs and to improve self-life of dosage form. Keywords: Hydroxyethyl cellulose; Hydroxypropyl cellulose; XRD; DSC; TGA; Biofield treatment; Abbreviation:HEC: Hydroxyethyl Cellulose; HPC: Hydroxypropyl Cellulose; XRD: X-ray Diffraction Study; DSC: Differential Scanning Calorimetry; TGA: Thermogravimetric Analysis; DTA: Differential Thermal Analysis; DDS: Drug Delivery System Introduction The oral route is by far the most preferred and convenient route for delivery of many pharmaceutically active drugs. Cellulose and cellulose based derivatives are accepted as natural materials with good tolerance by the human body and are commonly used in medical and pharmaceutical applications such as targeted DDS [4,5]. CHNSO analysis