The Biology Project: Cell Biology en español Cell Membranes Learn that membranes are fluid, with components that move, change, and perform vital physiological roles as they allow cells to communicate with each other and their environment. Cell Signaling Learn that living organisms constantly receive and interpret signals from their environment. Cells of multi-cellular organisms also receive signals from other cells, including signals for cell division and differentiation. Studying Cells Introduce yourself to the cell as the fundamental unit of life and the scientific method. The Cell Cycle & Mitosis Understand the events that occur in the cell cycle and the process of mitosis that divides the duplicated genetic material creating two identical daughter cells. Meiosis Understand the events that occur in process of meiosis that takes place to produce our gametes. Prokaryotes, Eukaryotes, & Viruses Learn about the cells that make up all living systems, their organelles, and the differences between living cells and viruses.
Five fingers of evolution - Paul Andersen In his talk, Paul Andersen explains the five causes of microevolution. Research one example for each cause in the human population. Use the following population simulator to simulate microevolution: Run the simulation using the default settings.
FISH pour le diagnostic préimplantatoire génétique This article describes the selection of suitable probes for single-cell FISH, spreading techniques for blastomere nuclei, and in situ hybridization and signal scoring, applied to pre-implantation genetic diagnosis (PGD) in a clinical setting. Date Published: 2/23/2011, Issue 48; doi: 10.3791/2570 Keywords: Medicine, Issue 48, Fluorescence in situ hybridization, Pre-implantation genetic diagnosis, PGD, Sex determination, Translocations, Chromosome aneuploidy Scriven, P. N., Kirby, T. L., Ogilvie, C. Pre-implantation genetic diagnosis (PGD) is an established alternative to pre-natal diagnosis, and involves selecting pre-implantation embryos from a cohort generated by assisted reproduction technology (ART). 1. Cell lysis buffer for spreading cells (0.2% Tween20 in 0.01 M HCl, pH 2.0) should be prepared 24 hours in advance and stored at -20°C Prepare 100 mL and filter 20 mL into a 30 mL sterile universal container using a sterile 20 mL syringe and syringe filter. 2. 3. 4. Figure 1. Figure 2.
Methods and protocols in molecular biology - GFP Applications Page designed by Wallace Marshall, Yale University. {*style:<b>Added: 28-Nov-1999 Hits: 982 Rating: 7.33 Votes: 3 [ Rate It ] </b>*} - Contains many molecular biology protocols as well as some Drosophila protocols. {*style:<b>Added: 26-Sep-2003 Hits: 447 Rating: 10 Votes: 1 - Data bases, systems, and programs on molecular biology and genetics. {*style:<b>Added: 22-Nov-1999 Hits: 897 Rating: 10.00 Votes: 1 - The following methods have been optimized and tested by the Molecular Biology Division of the Sequencing Unit over a period of several years: Construction of Organellar DNA Libraries, Preparation of Single-stranded DNA, Preparation of Plasmid DNA, DNA Sequencing Strategy, Sequencing Gel and Electrophoresis, Sequence Analysis. {*style:<b>Added: 1-Oct-1999 Hits: 1128 Rating: 0 Votes: 0
All Non-Africans Part Neanderthal, Genetics Confirm If your heritage is non-African, you are part Neanderthal, according to a new study in the July issue of Molecular Biology and Evolution. Discovery News has been reporting on human/Neanderthal interbreeding for some time now, so this latest research confirms earlier findings. Damian Labuda of the University of Montreal's Department of Pediatrics and the CHU Sainte-Justine Research Center conducted the study with his colleagues. They determined some of the human X chromosome originates from Neanderthals, but only in people of non-African heritage. "This confirms recent findings suggesting that the two populations interbred," Labuda was quoted as saying in a press release. The ancestors of Neanderthals left Africa about 400,000 to 800,000 years ago. Neanderthals possessed the gene for language and had sophisticated music, art and tool craftsmanship skills, so they must have not been all that unattractive to modern humans at the time.
How Water Shapes DNA Water molecules surround the genetic material DNA in a very specific way. Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) have discovered that, on the one hand, the texture of this hydration shell depends on the water content and, on the other hand, actually influences the structure of the genetic substance itself. These findings are not only important in understanding the biological function of DNA; they could also be used for the construction of new DNA-based materials. The DNA's double helix never occurs in isolation; instead, its entire surface is always covered by water molecules which attach themselves with the help of hydrogen bonds. But the DNA does not bind all molecules the same way. "We've been able to verify that some of the water is bound stronger whereas other molecules are less so," notes Dr. Analyses of the genetic material were conducted at the HZDR by the doctoral candidate Hassan Khesbak. "DNA is, thus, a responsive material," explains Karim Fahmy.
DAVID: Gene Functional Classification What does this tool do? Quickly translate given gene IDs to corresponding gene names in a batch way Provide links for each genes to DAVID Gene Report for in-depth information Search functionally related genes within user's input gene list or genome Key Concepts of "Search Related Genes" Any given gene is associating with a set of annotation terms. If genes share similar set of those terms (annotation profile), they are most likely involved in similar biological mechanisms. The algorithm adopts kappa statistics to quantitatively measure the degree of the agreement how genes share the ~75,000 annotation terms collected by DAVID knowledgebase. For any given gene(s), the tool instantly searches and lists the related genes passed kappa similarity measurement threshold. Find Related Genes Tool is very different and complementary to the common gene clustering methods, such as homologous genes based on sequence similarity; protein families based on one common biological activity.
Scientists observe single gene activity in living cells in detail for first time Researchers at Albert Einstein College of Medicine of Yeshiva University have for the first time observed the activity of a single gene in living cells. In an unprecedented study, published in the April 22 online edition of Science, Einstein scientists were able to follow, in real time, the process of gene transcription, which occurs when a gene converts its DNA information into molecules of messenger RNA (mRNA) that go on to make the protein coded by the gene. Robert Singer, Ph.D., co-director of the Gruss Lipper Biophotonics Center at Einstein and professor and co-chair of anatomy and structural biology, is senior author of the paper. The study's lead author is Daniel Larson, Ph.D., previously a member of Dr. Singer's lab and now an investigator at the National Cancer Institute and head of the institute's Systems Biology of Gene Expression Section. Using florescent proteins, the researchers were able to follow mRNA activity by inserting DNA sequences into a gene in live yeast cells.
PLoS Biology : Publishing science, accelerating research A Peer-Reviewed, Open Access Journal Current Issue PLOS Biology is a peer-reviewed, open-access journal featuring research articles of exceptional significance in all areas of biological science, from molecules to ecosystems.